|
WEEK |
DATE |
LECTURE |
SEMINAR |
PRACTICE |
|
1 |
September
5-9
. |
Proteins and bioenergetics: structure and function of proteins, thermodynamics of living systems
|
|
General information, work safety, principles of lab work (practical note) |
|
2 |
September
12-16. |
Enzymology: enzyme classes, coenzymes, characterisation of enzymes, isoenzymes, multienzyme systems
|
|
Determination of protein concentration
|
|
3 |
September
19-23. |
Enzymology: molecular mechanism of catalysis, enzyme kinetics, modulation and regulation of enzyme activity
|
|
Substrate specificity and temperature optimum of amylase enzyme activity (practical note) |
|
4 |
September
26 - 30 |
Carbohydrate metabolism: Digestion and absorption of carbohydrates, glycolysis, pyruvate dehydrogenase enzyme complex, gluconeogenesis
|
SEMINAR (proteins, enzymes)
|
|
|
5 |
October 3-7 |
Carbohydrate metabolism: Fructose and galactose metabolism, glycogen metabolism, pentose phosphate cycle and glucuronide shunt |
|
Assay of activity of alkaline phosphatase (practical note) |
|
6 |
October
10-14. |
Carbohydrate metabolism: regulation of blood glucose level, glycoproteins
Lipid metabolism: Eicosanoids, digestion and absorption of lipids, lipoprotein metabolism |
SEMINAR (carbohydrate metabolism)
|
|
|
7 |
October
17-21. |
Lipid metabolism: lipid mobilisation, oxidation of fatty acids, ketone bodies, diabetes mellitus
|
|
Determination of glucose-6-phosphatase activity (practical note)
turbidimetry and nephelometry (pdf format) |
|
8 |
October
24-28. |
Lipid metabolism: Synthesis of fatty acids, synthesis of triacyl glycerols and phospholipids, sphingolipids, cholesterol and steroid metabolism |
1st MTO
tests to practice (pdf format) |
VACATION
|
9 |
October
31 - November 4 |
VACATION |
|
|
|
10 |
November
7-11
. |
Amino acid metabolism: Digestion and absorption of proteins, catabolism of essential amino acids, fate of amino group, urea cycle |
SEMINAR (lipid metabolism)
scheme of lectures (lipids) (pdf format) |
|
|
11 |
November
14-18. |
Amino acid metabolism: metabolism of non-essential amino acids, fate of carbon skeleton of amino acids, one-carbon units, glutathione |
|
Determination of triacylglyerols and cholesterol |
|
12 |
November
21-25. |
Amino acid metabolism: Synthesis of hem and porphyrine, enterohepatic circulation of hem degradation products |
SEMINAR (amino acid metabolism)
scheme of lectures (amino acids) (pdf format)
2nd MTO |
|
|
13 |
November 28 - December 2 |
Citric acid cycle: steps and regulation of the cycle, relationship between the cycle and other metabolic pathways |
SEMINAR (citric acid cycle, respiratory chain, oxidative phosphorylation) nucleotide metabolism-patobiochemistry (pdf format) |
|
|
14 |
December
5-9
. |
Mitochondrial transport systems, mechanism of respiratory chain and oxidative phosphorylation |
|
Investigation of the oxygen consumption of isolated mitochondria (practical note) |
|
14 |
December
12-16. |
Nucleotide metabolism: synthesis and degradation of purine and pirimidine nucleotides, salvage pathways, synthesis of deoxyribonucleotides |
|
Nucleotide metabolism. Determination of uric acid concentration
determination of uric acid (pdf format) |
